{"id":387,"date":"2017-04-15T14:45:00","date_gmt":"2017-04-15T06:45:00","guid":{"rendered":"https:\/\/www.next-bioinfo.tw\/?p=387"},"modified":"2024-02-22T14:56:53","modified_gmt":"2024-02-22T06:56:53","slug":"%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e4%bd%bf%e7%94%a8seqtk%e5%b0%8dfastq%e9%99%8d%e6%8e%a1%e6%a8%a3","status":"publish","type":"post","link":"https:\/\/www.next-bioinfo.tw\/en\/2017\/04\/%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e4%bd%bf%e7%94%a8seqtk%e5%b0%8dfastq%e9%99%8d%e6%8e%a1%e6%a8%a3\/","title":{"rendered":"\u3010NGS \u6b21\u4e16\u4ee3\u57fa\u56e0\u9ad4\u8cc7\u6599\u79d1\u5b78\u3011\u4f7f\u7528seqtk\u5c0dFASTQ\u964d\u63a1\u6a23"},"content":{"rendered":"<p>\u5728\u4e00\u4e9bQC\u5de5\u4f5c\u7684\u6642\u5019\uff0c\u6211\u5011\u6703\u9700\u8981\u5f9e\u5b8c\u6574\u7684\u5b9a\u5e8f\u7d50\u679c\u4e2d\u62bd\u6a23\u4e00\u4e9breads\u4f86\u505a\u8655\u7406\u3002\u800cseqtk\u662f\u76ee\u524d\u6bd4\u8f03\u4e3b\u6d41\u7684tool\uff0c\u9019\u88e1\u7c21\u55ae\u4ecb\u7d39\u5982\u4f55\u5b89\u88dd\u548c\u4f7f\u7528\u5b83\u4f86\u505aFASTQ\u7684\u62bd\u6a23\u3002<\/p>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_54_1 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\" role=\"button\"><label for=\"item-6a6d40d009f9b\" ><span class=\"\"><span style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewbox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewbox=\"0 0 24 24\" version=\"1.2\" baseprofile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/label><input aria-label=\"Toggle\" type=\"checkbox\"  id=\"item-6a6d40d009f9b\"><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1' ><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/www.next-bioinfo.tw\/en\/2017\/04\/%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e4%bd%bf%e7%94%a8seqtk%e5%b0%8dfastq%e9%99%8d%e6%8e%a1%e6%a8%a3\/#%E4%B8%8B%E8%BC%89_%E7%B7%A8%E8%AD%AFseqtk\" title=\"\u4e0b\u8f09&amp;\u7de8\u8b6fseqtk\">\u4e0b\u8f09&amp;\u7de8\u8b6fseqtk<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/www.next-bioinfo.tw\/en\/2017\/04\/%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e4%bd%bf%e7%94%a8seqtk%e5%b0%8dfastq%e9%99%8d%e6%8e%a1%e6%a8%a3\/#%E7%94%A8seqtk%E9%99%8D%E6%8E%A1%E6%A8%A3PE_%E7%9A%84FASTQ\" title=\"\u7528seqtk\u964d\u63a1\u6a23PE \u7684FASTQ\">\u7528seqtk\u964d\u63a1\u6a23PE \u7684FASTQ<\/a><\/li><\/ul><\/nav><\/div>\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"%E4%B8%8B%E8%BC%89_%E7%B7%A8%E8%AD%AFseqtk\"><\/span>\u4e0b\u8f09&amp;\u7de8\u8b6fseqtk<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<div class=\"wp-block-kevinbatdorf-code-block-pro cbp-has-line-numbers\" data-code-block-pro-font-family=\"Code-Pro-JetBrains-Mono\" style=\"font-size:.875rem;font-family:Code-Pro-JetBrains-Mono,ui-monospace,SFMono-Regular,Menlo,Monaco,Consolas,monospace;--cbp-line-number-color:#d8dee9ff;--cbp-line-number-width:8.434028625488281px;line-height:1.25rem;--cbp-tab-width:2;tab-size:var(--cbp-tab-width, 2)\"><span role=\"button\" tabindex=\"0\" data-code=\"git clone https:\/\/github.com\/lh3\/seqtk.gitcd seqtkmake .\/seqtk\" style=\"color:#d8dee9ff;display:none\" aria-label=\"Copy\" class=\"code-block-pro-copy-button\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" style=\"width:24px;height:24px\" fill=\"none\" viewbox=\"0 0 24 24\" stroke=\"currentColor\" stroke-width=\"2\"><path class=\"with-check\" stroke-linecap=\"round\" stroke-linejoin=\"round\" d=\"M9 5H7a2 2 0 00-2 2v12a2 2 0 002 2h10a2 2 0 002-2V7a2 2 0 00-2-2h-2M9 5a2 2 0 002 2h2a2 2 0 002-2M9 5a2 2 0 012-2h2a2 2 0 012 2m-6 9l2 2 4-4\"><\/path><path class=\"without-check\" stroke-linecap=\"round\" stroke-linejoin=\"round\" d=\"M9 5H7a2 2 0 00-2 2v12a2 2 0 002 2h10a2 2 0 002-2V7a2 2 0 00-2-2h-2M9 5a2 2 0 002 2h2a2 2 0 002-2M9 5a2 2 0 012-2h2a2 2 0 012 2\"><\/path><\/svg><\/span><pre class=\"shiki nord\" style=\"background-color: #2e3440ff\" tabindex=\"0\"><code><span class=\"line\"><span style=\"color: #88C0D0\">git<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">clone<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">https:\/\/github.com\/lh3\/seqtk.git<\/span><\/span>\n<span class=\"line\"><span style=\"color: #88C0D0\">cd<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">seqtk<\/span><\/span>\n<span class=\"line\"><span style=\"color: #88C0D0\">make<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #88C0D0\">.\/seqtk<\/span><\/span><\/code><\/pre><\/div>\n\n\n\n<p>\u4e5f\u53ef\u4ee5\u7528conda\u5b89\u88dd\uff1a<\/p>\n\n\n\n<div class=\"wp-block-kevinbatdorf-code-block-pro cbp-has-line-numbers\" data-code-block-pro-font-family=\"Code-Pro-JetBrains-Mono\" style=\"font-size:.875rem;font-family:Code-Pro-JetBrains-Mono,ui-monospace,SFMono-Regular,Menlo,Monaco,Consolas,monospace;--cbp-line-number-color:#d8dee9ff;--cbp-line-number-width:8.434051513671875px;line-height:1.25rem;--cbp-tab-width:2;tab-size:var(--cbp-tab-width, 2)\"><span role=\"button\" tabindex=\"0\" data-code=\"conda install -c bioconda seqtk\" style=\"color:#d8dee9ff;display:none\" aria-label=\"Copy\" class=\"code-block-pro-copy-button\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" style=\"width:24px;height:24px\" fill=\"none\" viewbox=\"0 0 24 24\" stroke=\"currentColor\" stroke-width=\"2\"><path class=\"with-check\" stroke-linecap=\"round\" stroke-linejoin=\"round\" d=\"M9 5H7a2 2 0 00-2 2v12a2 2 0 002 2h10a2 2 0 002-2V7a2 2 0 00-2-2h-2M9 5a2 2 0 002 2h2a2 2 0 002-2M9 5a2 2 0 012-2h2a2 2 0 012 2m-6 9l2 2 4-4\"><\/path><path class=\"without-check\" stroke-linecap=\"round\" stroke-linejoin=\"round\" d=\"M9 5H7a2 2 0 00-2 2v12a2 2 0 002 2h10a2 2 0 002-2V7a2 2 0 00-2-2h-2M9 5a2 2 0 002 2h2a2 2 0 002-2M9 5a2 2 0 012-2h2a2 2 0 012 2\"><\/path><\/svg><\/span><pre class=\"shiki nord\" style=\"background-color: #2e3440ff\" tabindex=\"0\"><code><span class=\"line\"><span style=\"color: #88C0D0\">conda<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">install<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">-c<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">bioconda<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">seqtk<\/span><\/span><\/code><\/pre><\/div>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"%E7%94%A8seqtk%E9%99%8D%E6%8E%A1%E6%A8%A3PE_%E7%9A%84FASTQ\"><\/span>\u7528seqtk\u964d\u63a1\u6a23PE \u7684FASTQ<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>\u90fd\u53d610000\u500breads\uff1a<\/p>\n\n\n\n<div class=\"wp-block-kevinbatdorf-code-block-pro cbp-has-line-numbers\" data-code-block-pro-font-family=\"Code-Pro-JetBrains-Mono\" style=\"font-size:.875rem;font-family:Code-Pro-JetBrains-Mono,ui-monospace,SFMono-Regular,Menlo,Monaco,Consolas,monospace;--cbp-line-number-color:#d8dee9ff;--cbp-line-number-width:8.434051513671875px;line-height:1.25rem;--cbp-tab-width:2;tab-size:var(--cbp-tab-width, 2)\"><span role=\"button\" tabindex=\"0\" data-code=\".\/seqtk sample -s100 read1.fq 10000 &gt; sub1.fq.\/seqtk sample -s100 read2.fq 10000 &gt; sub2.fq\" style=\"color:#d8dee9ff;display:none\" aria-label=\"Copy\" class=\"code-block-pro-copy-button\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" style=\"width:24px;height:24px\" fill=\"none\" viewbox=\"0 0 24 24\" stroke=\"currentColor\" stroke-width=\"2\"><path class=\"with-check\" stroke-linecap=\"round\" stroke-linejoin=\"round\" d=\"M9 5H7a2 2 0 00-2 2v12a2 2 0 002 2h10a2 2 0 002-2V7a2 2 0 00-2-2h-2M9 5a2 2 0 002 2h2a2 2 0 002-2M9 5a2 2 0 012-2h2a2 2 0 012 2m-6 9l2 2 4-4\"><\/path><path class=\"without-check\" stroke-linecap=\"round\" stroke-linejoin=\"round\" d=\"M9 5H7a2 2 0 00-2 2v12a2 2 0 002 2h10a2 2 0 002-2V7a2 2 0 00-2-2h-2M9 5a2 2 0 002 2h2a2 2 0 002-2M9 5a2 2 0 012-2h2a2 2 0 012 2\"><\/path><\/svg><\/span><pre class=\"shiki nord\" style=\"background-color: #2e3440ff\" tabindex=\"0\"><code><span class=\"line\"><span style=\"color: #88C0D0\">.\/seqtk<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">sample<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">-s100<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">read1.fq<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #B48EAD\">10000<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">&gt;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">sub1.fq<\/span><\/span>\n<span class=\"line\"><span style=\"color: #88C0D0\">.\/seqtk<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">sample<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">-s100<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">read2.fq<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #B48EAD\">10000<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">&gt;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #A3BE8C\">sub2.fq<\/span><\/span><\/code><\/pre><\/div>\n\n\n\n<p>\u53c3\u8003\uff1ahttps:\/\/www.biostars.org\/p\/6544\/<\/p>\n\n\n\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>\u5728\u4e00\u4e9bQC\u5de5\u4f5c\u7684\u6642\u5019\uff0c\u6211\u5011\u6703\u9700\u8981\u5f9e\u5b8c\u6574\u7684\u5b9a\u5e8f\u7d50\u679c\u4e2d\u62bd\u6a23\u4e00\u4e9breads\u4f86\u505a\u8655\u7406\u3002\u800cseqtk\u662f\u76ee\u524d\u6bd4\u8f03\u4e3b\u6d41\u7684to [&hellip;]<\/p>","protected":false},"author":1,"featured_media":227,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-container-style":"default","site-container-layout":"default","site-sidebar-layout":"default","site-transparent-header":"default","disable-article-header":"default","disable-site-header":"default","disable-site-footer":"default","disable-content-area-spacing":"default","footnotes":""},"categories":[2],"tags":[17,51,50],"_links":{"self":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts\/387"}],"collection":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/comments?post=387"}],"version-history":[{"count":2,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts\/387\/revisions"}],"predecessor-version":[{"id":390,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts\/387\/revisions\/390"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/media\/227"}],"wp:attachment":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/media?parent=387"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/categories?post=387"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/tags?post=387"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}