{"id":355,"date":"2017-04-16T12:22:00","date_gmt":"2017-04-16T04:22:00","guid":{"rendered":"https:\/\/www.next-bioinfo.tw\/?p=355"},"modified":"2023-11-13T15:54:49","modified_gmt":"2023-11-13T07:54:49","slug":"%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e5%9f%ba%e7%a4%8e%e6%95%99%e5%ad%b8-06-%e6%9f%93%e8%89%b2%e9%ab%94%e9%95%b7%e5%ba%a6","status":"publish","type":"post","link":"https:\/\/www.next-bioinfo.tw\/en\/2017\/04\/%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e5%9f%ba%e7%a4%8e%e6%95%99%e5%ad%b8-06-%e6%9f%93%e8%89%b2%e9%ab%94%e9%95%b7%e5%ba%a6\/","title":{"rendered":"\u3010NGS \u6b21\u4e16\u4ee3\u57fa\u56e0\u9ad4\u8cc7\u6599\u79d1\u5b78\u3011\u57fa\u790e\u6559\u5b78 06 \u67d3\u8272\u9ad4\u9577\u5ea6\u8cc7\u8a0a.chrom.sizes\u7684\u4f7f\u7528"},"content":{"rendered":"

\u67d3\u8272\u9ad4\u9577\u5ea6\u8cc7\u8a0a\u662f\u5f88\u5e38\u898b\u65bc\u5206\u6790\u7a0b\u5f0f\u7576\u4e2d\u6703\u9700\u8981\u50b3\u5165\u7684\u8cc7\u6599\uff0c\u9019\u88e1\u7c21\u55ae\u8aaa\u660e\u5982\u4f55\u53d6\u5f97<\/p>\n\n\n\n

\u6c92\u6709\u7279\u6b8a\u9700\u6c42\u7684\u8a71\uff0c\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528IGV\u63d0\u4f9b\u7684\u73fe\u6210\u6578\u64da<\/p>\n\n\n\n

https:\/\/github.com\/igvteam\/igv\/tree\/master\/genomes\/sizes<\/a><\/p>\n\n\n\n

\u50cf\u662f\u5e38\u898b\u7684mm10<\/p>\n\n\n\n

https:\/\/github.com\/igvteam\/igv\/blob\/master\/genomes\/sizes\/mm10.chrom.sizes<\/a><\/p>\n\n\n\n

\u5c31\u53ef\u4ee5\u76f4\u63a5\u4e0b\u8f09<\/p>\n\n\n\n

<\/path><\/path><\/svg><\/span>
curl<\/span> <\/span>https:\/\/raw.githubusercontent.com\/igvteam\/igv\/master\/genomes\/sizes\/mm10.chrom.sizes<\/span> <\/span>-o<\/span> <\/span>mm10.chrom.sizes<\/span><\/span>\nhead<\/span> <\/span>mm10.chrom.sizes<\/span><\/span><\/code><\/pre><\/div>\n\n\n\n
\u6703\u5f97\u5230<\/p>\n\n\n\n
<\/path><\/path><\/svg><\/span>
chr1<\/span>\t<\/span>195471971<\/span><\/span>\nchr2<\/span>\t<\/span>182113224<\/span><\/span>\nchr3<\/span>\t<\/span>160039680<\/span><\/span>\nchr4<\/span>\t<\/span>156508116<\/span><\/span>\nchr5<\/span>\t<\/span>151834684<\/span><\/span>\nchr6<\/span>\t<\/span>149736546<\/span><\/span>\nchr7<\/span>\t<\/span>145441459<\/span><\/span>\nchr8<\/span>\t<\/span>129401213<\/span><\/span>\nchr9<\/span>\t<\/span>124595110<\/span><\/span>\nchr10<\/span>\t<\/span>130694993<\/span><\/span><\/code><\/pre><\/div>\n\n\n\n
\u6216\u662f\u53bbUCSC\u7684\u7db2\u7ad9\u4e0b\u8f09<\/p>\n\n\n\n
\u5982\u679c\u662f\u6c92\u6709\u63d0\u4f9b\u7684\uff0c\u5728\u624b\u4e0a\u6709\u53c3\u8003\u57fa\u56e0\u7d44\u7684\u539f\u59cb\u6a94FASTA(.fa)\u683c\u5f0f\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5\u7528samtools\u8a08\u7b97\uff1a<\/p>\n\n\n\n
<\/path><\/path><\/svg><\/span>
samtools<\/span> <\/span>faidx<\/span> <\/span>mm10.fa<\/span> <\/span>cut<\/span> <\/span>f1,2<\/span> <\/span>mm10.fa.fai<\/span> <\/span>><\/span> <\/span>mm10.chrom.sizes<\/span><\/span><\/code><\/pre><\/div>","protected":false},"excerpt":{"rendered":"
\u67d3\u8272\u9ad4\u9577\u5ea6\u8cc7\u8a0a\u662f\u5f88\u5e38\u898b\u65bc\u5206\u6790\u7a0b\u5f0f\u7576\u4e2d\u6703\u9700\u8981\u50b3\u5165\u7684\u8cc7\u6599\uff0c\u9019\u88e1\u7c21\u55ae\u8aaa\u660e\u5982\u4f55\u53d6\u5f97 \u6c92\u6709\u7279\u6b8a\u9700\u6c42\u7684\u8a71\uff0c\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528IG […]<\/p>","protected":false},"author":1,"featured_media":143,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-container-style":"default","site-container-layout":"default","site-sidebar-layout":"default","site-transparent-header":"default","disable-article-header":"default","disable-site-header":"default","disable-site-footer":"default","disable-content-area-spacing":"default","footnotes":""},"categories":[2],"tags":[19,43],"_links":{"self":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts\/355"}],"collection":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/comments?post=355"}],"version-history":[{"count":2,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts\/355\/revisions"}],"predecessor-version":[{"id":389,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts\/355\/revisions\/389"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/media\/143"}],"wp:attachment":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/media?parent=355"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/categories?post=355"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/tags?post=355"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
https:\/\/hgdownload.soe.ucsc.edu\/downloads.html<\/a><\/p>\n\n\n\n