{"id":271,"date":"2017-04-16T14:48:00","date_gmt":"2017-04-16T06:48:00","guid":{"rendered":"https:\/\/www.next-bioinfo.tw\/?p=271"},"modified":"2023-09-23T15:00:27","modified_gmt":"2023-09-23T07:00:27","slug":"%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e7%94%9f%e7%89%a9%e5%af%a6%e9%a9%97%e7%9a%84%e9%87%8d%e8%a4%87replicates","status":"publish","type":"post","link":"https:\/\/www.next-bioinfo.tw\/en\/2017\/04\/%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e7%94%9f%e7%89%a9%e5%af%a6%e9%a9%97%e7%9a%84%e9%87%8d%e8%a4%87replicates\/","title":{"rendered":"\u3010NGS \u6b21\u4e16\u4ee3\u57fa\u56e0\u9ad4\u8cc7\u6599\u79d1\u5b78\u3011\u751f\u7269\u5be6\u9a57\u7684\u91cd\u8907Replicates"},"content":{"rendered":"<p>Replicates \u662f\u6307\u540c\u6a23\u7684\u5be6\u9a57\u5728\u76f8\u540c\u689d\u4ef6\u4e4b\u4e0b\uff0c\u91cd\u8907\u5be6\u9a57\u7684\u7d50\u679c\u3002\u4e00\u822c\u751f\u91ab\u9818\u57df\u7684replicate\u53c8\u53ef\u5206\u70batechnical replicate\u548cbiological replicate\u3002<\/p>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_54_1 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\" role=\"button\"><label for=\"item-6a6d40c926728\" ><span class=\"\"><span style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewbox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewbox=\"0 0 24 24\" version=\"1.2\" baseprofile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/label><input aria-label=\"Toggle\" type=\"checkbox\"  id=\"item-6a6d40c926728\"><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1' ><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/www.next-bioinfo.tw\/en\/2017\/04\/%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e7%94%9f%e7%89%a9%e5%af%a6%e9%a9%97%e7%9a%84%e9%87%8d%e8%a4%87replicates\/#Technical_Replicate_%E6%8A%80%E8%A1%93%E6%80%A7%E9%87%8D%E8%A4%87\" title=\"Technical Replicate \u6280\u8853\u6027\u91cd\u8907\">Technical Replicate \u6280\u8853\u6027\u91cd\u8907<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/www.next-bioinfo.tw\/en\/2017\/04\/%e3%80%90ngs-%e6%ac%a1%e4%b8%96%e4%bb%a3%e5%9f%ba%e5%9b%a0%e9%ab%94%e8%b3%87%e6%96%99%e7%a7%91%e5%ad%b8%e3%80%91%e7%94%9f%e7%89%a9%e5%af%a6%e9%a9%97%e7%9a%84%e9%87%8d%e8%a4%87replicates\/#Biological_Replicate_%E7%94%9F%E7%89%A9%E6%80%A7%E9%87%8D%E8%A4%87\" title=\"Biological Replicate \u751f\u7269\u6027\u91cd\u8907\">Biological Replicate \u751f\u7269\u6027\u91cd\u8907<\/a><\/li><\/ul><\/nav><\/div>\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Technical_Replicate_%E6%8A%80%E8%A1%93%E6%80%A7%E9%87%8D%E8%A4%87\"><\/span>Technical Replicate \u6280\u8853\u6027\u91cd\u8907<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img decoding=\"async\" src=\"https:\/\/ml4fi34j3t2e.i.optimole.com\/w:1024\/h:421\/q:mauto\/f:avif\/https:\/\/www.next-bioinfo.tw\/wp-content\/uploads\/2023\/09\/pasted-image-0.png\" alt=\"\" class=\"wp-image-272\" style=\"width:513px;height:211px\" width=\"513\" height=\"211\"\/><\/figure>\n\n\n\n<p>\u662f\u6307\u751f\u7269\u5be6\u9a57\u7684\u5c0d\u8c61\u4e0d\u8b8a\uff0c\u4f8b\u5982\u5f9e\u540c\u4e00\u96bb\u5be6\u9a57\u9f20\u8eab\u4e0a\u7372\u5f97\uff0c\u4f46\u91cd\u8907\u9032\u884c\u79d1\u5b78\u6e2c\u91cf\u7684\u7d50\u679c(\u9019\u88e1\u7684\u79d1\u5b78\u6e2c\u91cf\u5176\u5be6\u662f\u4e00\u6574\u5957\u751f\u7269\u5be6\u9a57\u7684\u88fd\u5099\u6d41\u7a0b\uff0c\u53ef\u80fd\u6703\u5305\u542bsorting(\u7d30\u80de\u5206\u9078)\u3001culture(\u7d30\u80de\u57f9\u990a)\u3001RT-PCR \u5230\u88fd\u4f5c\u6210library\u7684\u5404\u7a2eSeq\u7684\u5404\u7a2e\u6d41\u7a0b)\uff0c\u505atechnical replicate \u53ef\u5354\u52a9\u9a57\u8b49protocol(\u5be6\u9a57\u914d\u65b9)\u7684\u518d\u73fe\u6027\u3002\u4ee5\u4e00\u500bdata scientist \u7684\u89d2\u5ea6\u4f86\u8aaa\u5c0d\u65bc\u53d6\u5f97\u7684technical replicates \u53ef\u4ee5\u7c21\u55ae\u8dd1correlation\u985e\u578b\u7684\u5206\u6790\u4f86\u5354\u52a9\u78ba\u8a8dtechnical replicate\u4e4b\u9593\u662f\u5426\u8db3\u5920\u76f8\u4f3c\uff0c\u53f3\u6216\u8005\u6703\u9700\u8981\u53bb\u8dd1\u4e0d\u540c\u7684protocol\u7684\u7d50\u679c\uff0c\u4e26\u5354\u52a9\u627e\u51fa\u8f03\u4f73\u7684\u65b9\u6848\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Biological_Replicate_%E7%94%9F%E7%89%A9%E6%80%A7%E9%87%8D%E8%A4%87\"><\/span>Biological Replicate \u751f\u7269\u6027\u91cd\u8907<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img decoding=\"async\" src=\"https:\/\/ml4fi34j3t2e.i.optimole.com\/w:1024\/h:469\/q:mauto\/f:avif\/https:\/\/www.next-bioinfo.tw\/wp-content\/uploads\/2023\/09\/pasted-image-0-1.png\" alt=\"\" class=\"wp-image-273\" style=\"width:501px;height:230px\" width=\"501\" height=\"230\"\/><\/figure>\n\n\n\n<p>\u662f\u6307\u751f\u7269\u5be6\u9a57\u7684\u5c0d\u8c61\u4f86\u6e90\u4e0d\u540c\u7684\u7d50\u679c\u3002\u53ef\u80fd\u662fN\u96bb\u4e0d\u540c\u7684\u5be6\u9a57\u9f20\uff0c\u6216N\u4f4ddonor(\u5be6\u9a57\u6a23\u672c\u7684\u6350\u8d08\u8005)\u3002biological replicate\u6703\u53d7\u5230\u4e0d\u540c\u7684\u5be6\u9a57\u7684\u5c0d\u8c61\u4e4b\u9593\u7684\u5dee\u7570(\u6709\u53ef\u80fd\u4f86\u81ea\u4e0d\u540c\u7684DNA)\u7684\u64fe\u52d5\uff0c\u4f46\u5982\u679c\u7814\u7a76\u767c\u73fe\u7684\u898f\u5f8b\u662f\u771f\u5be6\u7684\u8a71\uff0c\u7406\u8ad6\u4e0a\u61c9\u8a72\u8981\u80fd\u5728\u4e0d\u540cbiological replicate\u4e4b\u9593\u91cd\u8907\u8a72\u5be6\u9a57\u7684\u7d50\u679c\uff0c\u4e00\u822c\u800c\u8a00N=3\u53ef\u88ab\u63a5\u53d7\u3002<\/p>","protected":false},"excerpt":{"rendered":"<p>Replicates \u662f\u6307\u540c\u6a23\u7684\u5be6\u9a57\u5728\u76f8\u540c\u689d\u4ef6\u4e4b\u4e0b\uff0c\u91cd\u8907\u5be6\u9a57\u7684\u7d50\u679c\u3002\u4e00\u822c\u751f\u91ab\u9818\u57df\u7684replicate\u53c8\u53ef\u5206\u70bat [&hellip;]<\/p>","protected":false},"author":1,"featured_media":274,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-container-style":"default","site-container-layout":"default","site-sidebar-layout":"default","site-transparent-header":"default","disable-article-header":"default","disable-site-header":"default","disable-site-footer":"default","disable-content-area-spacing":"default","footnotes":""},"categories":[2],"tags":[30,28,29],"_links":{"self":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts\/271"}],"collection":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/comments?post=271"}],"version-history":[{"count":1,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts\/271\/revisions"}],"predecessor-version":[{"id":275,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/posts\/271\/revisions\/275"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/media\/274"}],"wp:attachment":[{"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/media?parent=271"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/categories?post=271"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.next-bioinfo.tw\/en\/wp-json\/wp\/v2\/tags?post=271"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}